Transport of B-Vitamins in Microorganisms. I. On the Permeability of Lactobacillus fermenti to 35S-Thiamine.

Abstract

The permeability of L. fermenti to s35-thiamine was studied using thiamine-deficient and thiamine-sufficient cells. Evidence was obtained that Mg2+ ions stimulate this permeability process, the uptake of S35-thiamine is markedly stimulated by glucose, the glucose stimulation is inhibited by iodoacetate, the iodoacetate inhibition can be reversed by adenosine triphosphate (ATP), ATP can to a considerable extent replace glucose as a permeability stimulator in thiamine-deficient cells, but is without effect on the sufficient ones.