A Particle Associated with the Polyadenylate Segment in Mammalian Messenger RNA
- 1 November 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (11), 3247-3250
- https://doi.org/10.1073/pnas.69.11.3247
Abstract
A structure consisting of poly(A) complexed with other components is released from polysomes by ribonuclease treatment. The poly(A) complex has a sedimentation value of 12-15, while the corresponding sedimentation value for free poly(A) is 4. The complex does not appear to represent an artifact formed by interaction of free poly(A) with either cytoplasmic or polysomal proteins. The polynucleotide released from the complex by treatment with sodium dodecyl sulfate shows the same electrophoretic mobility as that of poly(A) isolated from deproteinized polysomal RNA. The poly(A) in the complex is partially protected from digestion by T(2) ribonuclease. At least part of the poly(A) is available for base pairing with poly(U). The components associated with the poly(A) cause it to bind to Millipore filters at low ionic strength. These components are removed from the complex by Pronase digestion. The findings indicate that the poly(A) segment in messenger RNA serves as a binding site for a particle. This particle appears to consist of proteins.Keywords
This publication has 16 references indexed in Scilit:
- The localization of polyadenylic sequence at the 5′-end of light nuclear dRNAFEBS Letters, 1972
- Isolation of mammalian messenger ribonucleic acidBiochemistry, 1972
- Polyadenylic Acid Sequences in the Heterogeneous Nuclear RNA and Rapidly-Labeled Polyribosomal RNA of HeLa Cells: Possible Evidence for a Precursor RelationshipProceedings of the National Academy of Sciences, 1971
- Pulse-labeled ribonucleic acid complexes released by dissociation of rat liver polysomesBiochemistry, 1971
- Adenine-rich Polymer associated with Rabbit Reticulocyte Messenger RNANature, 1970
- Rapidly labeled, polydisperse RNA in rat-liver cytoplasm: Evidence that it is contained in ribonucleoprotein particles of heterogeneous sizeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromideJournal of Molecular Biology, 1969
- Messenger RNA in rat liver polyribosomes: Evidence that it exists as ribonucleoprotein particlesJournal of Molecular Biology, 1968
- Messenger RNA-protein complexes and newly synthesized ribosomal subunits: Analysis of free particles and components of polyribosomesJournal of Molecular Biology, 1968
- An acrylamide gel soluble in scintillation fluid: Its application to electrophoresis at neutral and low pHAnalytical Biochemistry, 1965