Differential Arterial/Venous Expression of NG2 Proteoglycan in Perivascular Cells Along Microvessels: Identifying a Venule‐Specific Phenotype
- 1 March 2005
- journal article
- Published by Wiley in Microcirculation
- Vol. 12 (2), 151-160
- https://doi.org/10.1080/10739680590904955
Abstract
Objective: Similar to other vascular pericyte markers, including smooth muscle (SM) α‐actin, desmin, and PDGF‐β‐receptor, NG2 proteoglycan is not pericyte specific. Therefore, the use of NG2 as a pericyte marker, especially in cell lineage studies, in comparison to other nonspecific pericyte markers requires an understanding of how its expression varies spatially within a microvascular network. The objective of this study was to characterize NG2 expression along vessels within rat microvascular networks and compare this to SM α‐actin expression. Methods: Mesenteric tissue, subcutaneous tissue, spinotrapezius muscle, and gracilis muscle were harvested from 250‐g, female, Sprague–Dawley rats and stained for NG2 and SM α‐actin. The distribution of NG2 expression was evaluated in mesenteric networks (n = 28) with complementary observations in subcutaneous tissue and skeletal muscle. Results: Perivascular cells, including mature smooth muscle cells (SMCs), immature SMCs, and pericytes, expressed NG2. Most importantly, NG2 expression was primarily confined to perivascular cells along arterioles and capillaries, and continuous expression was not observed along venules beyond the immediate postcapillary vessels. The differential expression of NG2 along the arteriolar side of microvascular networks was also observed in rat subcutaneous and skeletal muscle. Conclusions: The results indicate that NG2 is expressed by all perivascular cells along arterioles, and its absence denotes a venule‐specific phenotype. These results identify for the first time a marker that differentiates venous smooth muscle and pericytes from other capillary‐ and arteriole‐associated perivascular cells.Keywords
This publication has 31 references indexed in Scilit:
- EphB receptors and ephrinB ligands: regulators of vascular assembly and homeostasisCell and tissue research, 2003
- Endothelial-pericyte interactions in angiogenesisCell and tissue research, 2003
- Enhanced Smooth Muscle Cell Coverage of Microvessels Exposed to Increased Hemodynamic Stresses In VivoCirculation Research, 2003
- The Proteoglycan NG2 Is Complexed with α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors by the PDZ Glutamate Receptor Interaction Protein (GRIP) in Glial Progenitor CellsJournal of Biological Chemistry, 2003
- Arteries and veins: making a difference with zebrafishNature Reviews Genetics, 2002
- Gridlock signalling pathway fashions the first embryonic arteryNature, 2001
- Binding of the NG2 Proteoglycan to Kringle Domains Modulates the Functional Properties of Angiostatin and Plasmin(ogen)Journal of Biological Chemistry, 2000
- Participation of the NG2 Proteoglycan in Rat Aortic Smooth Muscle Cell Responses to Platelet-Derived Growth FactorExperimental Cell Research, 1995
- Chondroitin sulfate proteoglycans in the developing cerebral cortex: The distribution of neurocan distinguishes forming afferent and efferent axonal pathwaysJournal of Comparative Neurology, 1995
- The primary structure of NG2, a novel membrane-spanning proteoglycan.The Journal of cell biology, 1991