Complete, 12-subunit RNA polymerase II at 4.1-Å resolution: Implications for the initiation of transcription
Open Access
- 13 May 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 100 (12), 6969-6973
- https://doi.org/10.1073/pnas.1130601100
Abstract
The x-ray structure of complete RNA polymerase II from Saccharomyces cerevisiae has been determined, including a heterodimer of subunits Rpb4 and Rpb7 not present in previous “core” polymerase II structures. The heterodimer maintains the polymerase in the conformation of a transcribing complex, may bind RNA as it emerges from the enzyme, and is in a position to interact with general transcription factors and the Mediator of transcriptional regulation.Keywords
This publication has 41 references indexed in Scilit:
- Functional profiling of the Saccharomyces cerevisiae genomeNature, 2002
- Deletion of the RNA Polymerase Subunit RPB4 Acts as a Global, Not Stress-specific, Shut-off Switch for RNA Polymerase II Transcription at High TemperaturesPublished by Elsevier ,2001
- Evidence That Gal11 Protein Is a Target of the Gal4 Activation Domain in the MediatorBiochemistry, 2001
- An hsRPB4/7-dependent yeast assay for trans-activation by the EWS oncogeneOncogene, 2001
- Dissociable Rpb4-Rpb7 Subassembly of RNA Polymerase II Binds to Single-strand Nucleic Acid and Mediates a Post-recruitment Step in Transcription InitiationJournal of Biological Chemistry, 2001
- Mediator of Transcriptional RegulationAnnual Review of Biochemistry, 2000
- Yeast RNA Polymerase II Transcription Reconstituted with Purified ProteinsMethods, 1997
- [20] Processing of X-ray diffraction data collected in oscillation modeMethods in Enzymology, 1997
- SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modelingElectrophoresis, 1997
- A Minimal Set of RNA Polymerase II Transcription Protein InteractionsPublished by Elsevier ,1996