Reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype
- 1 November 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 60 (2), 491-496
- https://doi.org/10.1128/jvi.60.2.491-496.1986
Abstract
Genetic studies of reassortant rotaviruses have demonstrated that gene segments 4 and 9 each segregate with the serotype-specific neutralization phenotype in vitro. Reassortant rotaviruses derived by coinfection of MA-104 cells with the simian strain SA11 and the antigenically distinct bovine strain NCDV were used to determine which viral genes coded for proteins which induced a protective immune response in vivo. In addition, reassortant rotaviruses containing only the gene segment 4 or 9 protein products (vp3 and vp7, respectively) from SA11 or NCDV were used to determine the serotypic specificities of both vp3 and vp7 in several mammalian rotavirus strains. vp3 and vp7 from the murine strain Eb were shown to be indistinguishable from the corresponding proteins from strain SA11. Adult mice orally inoculated with strain Eb developed neutralizing antibodies to both vp3 and vp7. The two naturally occurring bovine rotavirus strains NCDV and UK were shown to contain antigenically similar vp7 but distinct vp3 proteins. Mouse dams orally immunized with a reassortant virus containing only gene 9 from NCDV passively protected their progeny against UK challenge, whereas mouse dams orally immunized with a reassortant virus containing only gene 4 from NCDV did not. Finally, we constructed reassortant viruses that immunized against rotaviruses of two distinct serotypes. SA11 .times. NCDV reassortants that contained vp3 and vp7 from different parents induced a protective immune response against both parental serotypes. vp3 and vp7 were independently capable of inducing a protective immune response after oral immunization. An understanding of the serotypic specificities of both vp3 and vp7 of human rotavirus isolates will be necessary for the development of successful strategies to protect infants against severe rotavirus infections.This publication has 32 references indexed in Scilit:
- Preparation and Characterization of Neutralizing Monoclonal Antibodies with Different Reactivity Patterns to Human RotavirusesJournal of General Virology, 1985
- Extragenic suppression of temperature-sensitive phenotype in reovirus: Mapping suppressor mutationsVirology, 1984
- Serotypic Similarity and Diversity of Rotaviruses of Mammalian and Avian Origin as Studied by Plaque-Reduction NeutralizationThe Journal of Infectious Diseases, 1984
- IMMUNOGENICITY AND SAFETY OF LIVE ORAL ATTENUATED BOVINE ROTAVIRUS VACCINE STRAIN RIT 4237 IN ADULTS AND YOUNG CHILDRENThe Lancet, 1983
- The cultivation of human rotavirus, strain ‘Wa’, to high titer in cell culture and characterization of the viral structural polypeptidesJournal of Virological Methods, 1983
- Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formationVirology, 1983
- Rotaviruses: A ReviewPublished by Springer Nature ,1983
- Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigensVirology, 1981
- A Two-Year Study of Bacterial, Viral, and Parasitic Agents Associated with Diarrhea in Rural BangladeshThe Journal of Infectious Diseases, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970