Fragmentation analysis of normal and dystrophic avian muscle
- 1 May 1982
- journal article
- research article
- Published by Wiley in Muscle & Nerve
- Vol. 5 (5), 373-381
- https://doi.org/10.1002/mus.880050507
Abstract
A difference between normal and dystrophic avian muscle was demonstrated by comparing the patterns of fragmentation of muscle during homogenization. Fragmentation was monitored by morphological methods and by viscometry. This method of fragmentation analysis depends on the principle that the viscosity of a suspension is an exponential function of the partial volume fraction occupied by the suspended particles; the more a tissue is fragmented into smaller pieces, the greater the viscosity of the resulting homogenate. Increasing the duration of homogenization of either fresh muscle in buffer or glycerinated muscle in relaxing solution gradually increased the viscosity of the homogenate of normal muscle, whereas the viscosity of the homogenate of dystrophic muscle remained approximately constant. This difference in viscosity indicated that dystrophic muscle was sheared into larger pieces which were resistant to further fragmentation. Electron microscopic examination showed that the homogenate of dystrophic muscle contained rows of sarcomeres, whereas normal muscle was sheared into amorphous masses of myofilaments.This publication has 14 references indexed in Scilit:
- In Vivo Effects of Protease Inhibitors on Chickens with Hereditary Muscular DystrophyJCI Insight, 1981
- Abnormal myosin heavy chain variant associated with avian muscular dystrophyCell Motility, 1981
- Intermediate filaments as mechanical integrators of cellular spaceNature, 1980
- Early detection of inherited muscular dystrophy in chickensCell and tissue research, 1979
- PHOSPHODIESTERS IN MUSCULAR DYSTROPHIES *Annals of the New York Academy of Sciences, 1979
- RELEVANCE OF GENETIC ANIMAL MODELS OF MUSCULAR DYSTROPHY TO HUMAN MUSCULAR DYSTROPHIES *Annals of the New York Academy of Sciences, 1979
- MAJOR PHYSIOLOGIC AND HISTOCHEMICAL CHARACTERISTICS OF INHERITED DYSTROPHY OF THE CHICKEN *Annals of the New York Academy of Sciences, 1979
- New elastic protein from muscleNature, 1976
- The role of actin in the temperature-dependent gelation and contraction of extracts of Acanthamoeba.The Journal of cell biology, 1976
- Stages in fibre breakdown in duchenne muscular dystrophy: An electron-microscopic studyJournal of the Neurological Sciences, 1975