Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones.

Abstract

Alloreactive mouse T cell clones with distinct specificities were use to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone but not other clones. The active fraction of the antisera for T cell proliferation was Ig. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in this assay, or their effect may be produced by anti-idiotype. Thus, anti-idiotype may provide .gtoreq. 2 specific T cell signals.