Enzyme Measurements on Single Cells
- 31 May 1974
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 184 (4140), 992-994
- https://doi.org/10.1126/science.184.4140.992
Abstract
A simple and sensitive procedure has been developed for enzymatic assays in single cells and applied to the measurement of β-glucuronidase in single parenchymal cells of liver. Cells deposited in microdroplets under oil were ruptured by freezing and thawing and allowed to react with a fluorogenic substrate. The fluorescence is developed by diffusing an organic base into the droplets and measured in a fluorescence microscope equipped with a photomultiplier.Keywords
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