Thermal Degradation of Foot-and-Mouth Disease Virus Into Infectious Ribonucleic Acid.
- 1 July 1961
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 107 (3), 610-613
- https://doi.org/10.3181/00379727-107-26703
Abstract
This virus degrades upon heating at 61[degree] or 85[degree]C into infectious ribonucleic acid (RNA) provided that environmental ribonuclease (RNase) is either inactivated by hot sodium dodecylsulfate or is completely removed prior to heating. Heat alone is sufficient to produce infectious RNA, and hot detergent only serves to inactivate RNase. Phenol treatment of heat-derived infectious RNA did not increase the amount of infectious RNA present. An earlier hypothesis is negated that boiling at 100[degree]C in 0.1% SDS only inactivates an infection-initiating property of the virus protein, leaving a phenol-extractable infectious core. The newer interpretation is that thermally-released RNA is not completely inactivated during 5 minutes boiling in detergent.Keywords
This publication has 5 references indexed in Scilit:
- Ribonuclease contamination of crystalline deoxyribonuclease, trypsin, and of partially purified foot-and-mouth disease virus preparationsAnalytical Biochemistry, 1961
- A Biological Assay for Ribonuclease with Sub-millimicrogram SensitivityExperimental Biology and Medicine, 1960
- Ribonucleic acid of foot-and-mouth disease virus: Its preparation, stability, and plating efficiency on bovine-kidney culturesVirology, 1960
- Some Applications of Detergents to the Study of the Virus of Foot-and-Mouth DiseaseJournal of General Microbiology, 1959
- Inactivation of Foot-and-Mouth Disease Virus by pH and Temperature Changes and by FormaldehydeExperimental Biology and Medicine, 1957