Gibberellic Acid-enhanced Release of β-1,3-Glucanase from Barley Aleurone Cells
- 1 March 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 47 (3), 412-416
- https://doi.org/10.1104/pp.47.3.412
Abstract
A beta-1, 3-glucanase of barley (Hordeum vulgare) aleurone cells accumulates when half-seeds are imbibed on water, and accumulation continues when the aleurone layers are incubated in buffer solution. The release of the enzyme is a gibberellic acid-dependent process, however. Although gibberellic acid stimulates glucanase release, it does not markedly affect the total amount of glucanase obtained from these cells when compared with water controls. beta-1, 3-Glucanase release from aleurone cells is a function of gibberellic acid concentration and commences after a 4-hour lag period. Processes occurring during this lag period are also dependent upon gibberellic acid concentration. Removal of gibberellic acid from the incubation medium at the end of the lag period, however, does not affect subsequent release of glucanase. The release of glucanase from aleurone cells is an active process with a Q(10) greater than 3. Inhibitors of respiration and protein and RNA synthesis effectively inhibit the formation and release of glucanase. It is concluded that gibberellic acid functions primarily to enhance glucanase release rather than its formation.Keywords
This publication has 4 references indexed in Scilit:
- Gibberellic Acid-Induced Synthesis of Protease by Isolated Aleurone Layers of BarleyPlant Physiology, 1967
- A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.Proceedings of the National Academy of Sciences, 1967
- Hormonal Control of Enzyme Synthesis: On the Mode of Action of Gibberellic Acid and Abscisin in Aleurone Layers of BarleyPlant Physiology, 1967
- Gibberellic Acid-Enhanced Synthesis and Release of α-Amylase and Ribonuclease by Isolated Barley and Aleurone LayersPlant Physiology, 1967