Changes in muscle lipid composition and resistance adaptation to temperature in the freshwater crayfish,Austropotamobius pallipes
- 1 April 1976
- Vol. 11 (4), 307-316
- https://doi.org/10.1007/bf02544059
Abstract
The phospholipid and fatty acid composition of muscle lipid extracts from crayfish acclimated to 4 C and 25 C (18 hr‐light photoperiod) were analyzed. The phospholipid content and class distribution, and cholesterol content were unaffected by the acclimation treatment. Unsaturation of muscle phosphoglycerides was higher in cold acclimated crayfish. Serine/inositol phosphoglycerides from cold‐acclimated animals showed somewhat higher proportions of mono‐and polyunsaturated fatty acids, whereas choline and ethanolamine phosphoglycerides were less affected. This was correlated with a decreased resistance of cold‐acclimated crayfish to lethal high temperature. Acclimation at 4 C under an 8 hr‐light photoperiod caused an increased fatty acid unsaturation of the total phospholipid fraction compared to the 4 C, 18 hr‐light photoperiod acclimated animals. The resistance of 4 C acclimated crayfish to lethal high temperature, however, was unaffected by daylength treatment. The resistance of freshwater crayfish to lethal high temperature is not simply related to the degree of saturation of the muscle phospholipids. It is suggested that a breakdown in the integrity of a bulk‐lipid bilayer is not involved in the process of heat death; rather, that a membrane‐bound protein factor, whose thermal sensitivity is modified by changes in its phospholipid environment during temperature adaptation but not during photoperiod adaptation, is the primary site of heat injury.This publication has 50 references indexed in Scilit:
- Heat death in the crayfish Austropotamobius pallipes: Thermal inactivation of muscle membrane-bound atpases in warm and cold adapted animalsJournal of Thermal Biology, 1976
- Heat death in the crayfish Austropotamobius pallipes— Ion movements and their effects on excitable tissues during heat deathJournal of Thermal Biology, 1976
- Glycerophosphatide content and composition of trout (Salvelinus fontinalis M.) brainJournal of Thermal Biology, 1975
- Chromatography of lipids in presence of an antioxidant, 4-methyl-2,6-di-tert.-butylphenolJournal of Chromatography A, 1964
- Detection of volume parts per million of permanent gases in heliumJournal of Chromatography A, 1964
- Brain lipid fatty acids and temperature acclimationComparative Biochemistry and Physiology, 1964
- An analysis of separation factors applicable in the gas‐liquid chromatography of unsaturated fatty acid methyl esters on a polyester substrateJournal of Oil & Fat Industries, 1963
- Structural correlation of unsaturated fatty acid esters through graphical comparison of gas‐liquid chromatographic retention times on a polyester substrateJournal of Oil & Fat Industries, 1963
- A simple method for the determination of serum cholesterolClinica Chimica Acta; International Journal of Clinical Chemistry, 1960
- EFFECTS OF THERMAL CONDITIONING AND OF DEGREE OF SATURATION OF DIETARY LIPIDS ON RESISTANCE OF AN INSECT TO A HIGH TEMPERATURECanadian Journal of Zoology, 1958