Cellular damage associated with multiple mating inE. coli

Abstract

In crosses in which the ratio of Hfr to F- cells was varied it was found that with one group of Hfr strains the yield of recombinants increased linearly with donor concentration until the number of donor and recipient cells were approximately equal. With other Hfr strains marked deviations from linearity were observed at Hfr: F⁻ratios greater than 1:5 and in crosses with one particular F⁻strain the yield of recombinants actually decreased progressively as the Hfr concentration was raised beyond this value. The deviations from linearity observed with this group of strains have been shown to be due to damage to F⁻cells which mate simultaneously with several Hfr cells.