Identification of LDL receptor gene marker associated with altered levels of LDL cholesterol and apolipoprotein B in baboons.
- 1 November 1989
- journal article
- research article
- Published by Wolters Kluwer Health in Arteriosclerosis: An Official Journal of the American Heart Association, Inc.
- Vol. 9 (6), 829-835
- https://doi.org/10.1161/01.atv.9.6.829
Abstract
We are studying the genetic factors underlying the common forms of heart disease by identifying genes that affect normal variations in plasma lipid, lipoprotein, and apolipoprotein concentrations in baboons. For these studies we are using cloned human gene probes to identify restriction fragment length polymorphisms (RFLPs) at loci encoding the proteins of cholesterol metabolism. In this report we present the identification and mapping of a polymorphic Ava II cleavage site in intron 17 of the baboon low density lipoprotein (LDL) receptor gene. We determined genotypes for this RFLP on a population of 253 pedigreed baboons and assessed the effect of LDL receptor RFLP genotypes on serum concentrations of LDL cholesterol (LDL-C) and apolipoprotein B (apo B). These measures were obtained for each baboon on each of two diets: a low cholesterol, low fat (basal) diet and a high cholesterol, high saturated fat (atherogenic) diet. Statistical analysis detected a significant association between LDL receptor genotype and serum LDL-C and apo B concentrations on both diets. Homozygotes for the rarer allele had lower serum concentrations of LDL-C and apo B than did homozygotes for the common allele, and with the exception of apo B levels on the basal diet, intermediate levels were observed in heterozygotes. The LDL receptor RFLP accounted for approximately 3% to 7% of the variation in serum LDL-C and apo B concentrations on both diets.This publication has 15 references indexed in Scilit:
- The use of measured genotype information in the analysis of quantitative phenotypes in man. II. The role of the apolipoprotein E polymorphism in determining levels, variability, and covariability of cholesterol, betalipoprotein, and triglycerides in a sample of unrelated individualsAmerican Journal of Medical Genetics, 1987
- A COMMON DNA POLYMORPHISM OF THE LOW-DENSITY LIPOPROTEIN (LDL) RECEPTOR GENE AND ITS USE IN DIAGNOSISThe Lancet, 1985
- The human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNACell, 1984
- Effects of selection for serum cholesterol concentrations on serum lipid concentrations and body weight in baboonsAmerican Journal of Medical Genetics, 1984
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Influence of infant and juvenile diets on serum cholesterol, lipoprotein cholesterol, and apolipoprotein concentrations in juvenile baboons ( sp.)Atherosclerosis, 1982
- Relationship of lipoprotein cholesterol concentrations to experimental atherosclerosis in baboons.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1981
- Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.Proceedings of the National Academy of Sciences, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodiesAnalytical Biochemistry, 1966