Dissimilarity of Changes Induced in Absorption Spectrum of 2-(4'-Hydroxyphenylazo)-Benzoic Acid by Different Serum Albumins

Abstract

Summary Addition of different serum albumins to solutions of the dye 2-(4′-hydroxyphenylazo)-benzoic acid caused widely different changes in the absorption spectrum of the dye. The changes differed in pattern as well as in magnitude, being largely independent of the quantitative differences in dye binding displayed by the different albumins. Different preparations of the individual albumins induced dye absorption changes sufficiently consistent and distinctive that each of the albumins could be distinguished from most of the others.