ELECTRON TRANSPORT PARTICLES FROM BACILLUS STEAROTHERMOPHILUS

Abstract
Electron-transport particles (ETP) have been isolated from Bacillus stearothermophilus. They are capable of oxidizing such substrates as succinate, malate, diphosphopyridine nucleotide, p-phenylenediamine and hydroquinone. Difference spectra indicated bands of cytochromes a3, b and c. A chromatographic procedure for purification of cytochrome c has been described. The role of quinonelike intermediates in the EPT was implied by the restorative effect of coenzyme Q and vitamin K1 on material treated with lipid solvent. Isolation and identification of coenzyme Q from the thermophile indicated it was similar to coenzyme Q10 of mammalian sources.

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