Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids
- 1 January 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (1), 468-470
- https://doi.org/10.1128/jb.170.1.468-470.1988
Abstract
A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn10, which is lethal when in multiple copies in the presence of 25 micrograms of tetracycline per ml.This publication has 29 references indexed in Scilit:
- Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) geneJournal of Bacteriology, 1987
- Cloning of genes from members of the family Enterobacteriaceae with mini-Mu bacteriophage containing plasmid repliconsJournal of Bacteriology, 1987
- Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusingJournal of Bacteriology, 1986
- In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.Proceedings of the National Academy of Sciences, 1984
- Resistance to the TetracyclinesPublished by Elsevier ,1984
- Phage Mu: Transposition as a Life-StylePublished by Elsevier ,1983
- Nucleotide sequence of the Tn10 encoded tetracycline resistance geneNucleic Acids Research, 1983
- Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase CJournal of Bacteriology, 1982
- Genetic organization of transposon Tn10Cell, 1981
- Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.Proceedings of the National Academy of Sciences, 1975