Maitotoxin stimulates phosphoinositide breakdown in neuroblastoma hybrid NCB-20 cells
- 1 September 1987
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Neurobiology
- Vol. 7 (3), 317-322
- https://doi.org/10.1007/bf00711308
Abstract
Maitotoxin (MTX) was an extraordinarily potent stimulant of phosphoinositide breakdown in the neuroblastoma hybrid NCB-20 cells. Maximal responses were obtained at 0.25–0.5 ng MTX/ml, and resulted in increased formation of [3H]inositol mono-, bis-, and trisphosphates. Increased formation of [3H]inositol bis- and trisphosphate was observed as early as 15 sec after the addition of MTX. MTX-induced phosphoinositide breakdown in NCB-20 cells was not antagonized by organic (nifedipine, methoxyverapamil) or inorganic (Mn2+, Co2+, Cd2+) calcium channel blockers. However, the response on phosphoinositide breakdown was completely eliminated in the absence of extracellular calcium. The results suggest that MTX either directly stimulates phosphoinositide breakdown in a calcium-dependent manner or acts indirectly through calcium channels insensitive to organic/inorganic calcium channel blockers.This publication has 14 references indexed in Scilit:
- Maitotoxin‐Induced Release of γ‐[3H]Aminobutyric Acid from Cultures of Striatal NeuronsJournal of Neurochemistry, 1986
- Carbachol-induced accumulation of inositol-1-phosphate in neurohybridoma NCB-20 cells: Effects of lithium and phorbol estersBiochemical and Biophysical Research Communications, 1986
- Maitotoxin stimulat es the formation of inositol phosphates in rat aortic myocytesFEBS Letters, 1986
- The mechanism of action of maitotoxin in relation to Ca2+ movements in guinea‐pig and rat cardiac musclesBritish Journal of Pharmacology, 1985
- Interactions of maitotoxin with voltage-sensitive calcium channels in cultured neuronal cells.Proceedings of the National Academy of Sciences, 1984
- Potent excitatory effect of maitotoxin on Ca channels in the insect skeletal musclePflügers Archiv - European Journal of Physiology, 1984
- Effects of ciguatoxin and maitotoxin on isolated rat atria and rabbit duodenumToxicon, 1984
- Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositidesBiochemical Journal, 1983
- Contractile response of the rabbit aorta to maitotoxin, the most potent marine toxin.The Journal of Physiology, 1983
- Genetic Dissection of Neural Properties using Somatic Cell HybridsNature New Biology, 1972