Structural Organization and Unusual Codon Usage in the DNA Polymerase Gene from Herpes Simplex Virus Type 1
- 1 August 1986
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 5 (4), 281-288
- https://doi.org/10.1089/dna.1986.5.281
Abstract
We have analyzed the protein and nucleic acid sequences of the DNA polymerase from herpes simplex virus type 1 (HSV-1) to provide insight into the expression and possible structure of this enzyme. Extensive similarity between the amino acid sequence and that of the Epstein Barr virus DNA polymerase is reported. We describe probable structural similarities between these proteins and the use of these similarities to define structural and functional domains within the polymerase. Analysis of base composition and codon usage reveals that several genes from HSV-1, including DNA polymerase, exhibit a strong preference for guanine or cytosine at the third codon position. This preference may result from the high guanine + cytosine content of the virus and produces a highly restricted codon usage, different from that of the host cell. Consequences of the unusual codon usage for viral expression include the potential for extensive mRNA secondary structure.This publication has 40 references indexed in Scilit:
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiencyCell, 1985
- Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymeraseNature, 1985
- Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMPNature, 1985
- Generation of genetic diversity in herpes simplex virus: An antimutator phenotype maps to the DNA polymerase locusVirology, 1984
- DNA METHYLATION AND GENE ACTIVITYAnnual Review of Biochemistry, 1983
- DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)Journal of General Virology, 1982
- GENOME VARIATION AND EVOLUTION AMONG HERPES VIRUSES*Annals of the New York Academy of Sciences, 1980
- Globin mRNA Sequences: Analysis of Base Pairing and Evolutionary ImplicationsCold Spring Harbor Symposia on Quantitative Biology, 1978
- Mode of inhibition of Herpes simplex virus DNA polymerase by phosphonoacetateBiochemistry, 1975