Effect of Age on Brain Cortical Protein Kinase C and Its Mediation of 5‐Hydroxytryptamine Release
- 1 January 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 52 (1), 187-192
- https://doi.org/10.1111/j.1471-4159.1989.tb10915.x
Abstract
The effects of age on the activity and translocation of protein kinase C (PKC) and on the facilitation of 5-hydroxytryptamine (5-HT, serotonin) release induced by PKC activation with the phorbol ester phorbol 12-myristate 13-acetate were investigated. The activities of cortical PKC and its translocation in response to K+ depolarization and phorbol ester stimulation were reduced during aging in Fischer-344 rats. Parietal cortical brain slices from 6-, 12-, and 24-month-old animals were preloaded with [3H]5-HT and release was evoked by 65 mM K+ or the calcium ionophore A23187. 5-HT release induced by either K+ or A23187 was found to be reduced in 12- and 24-month-old as compared to 6-months-old animals. This decrease was not reversed by high extra-cellular Ca2+. Activation of PKC resulted in a facilitated transmitter release in tissue from 6- and 12-month-old animals but reduced [3H]5-HT release in slices from 24-month-old animals. These responses were prevented by the putative PKC inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), but not by increasing extracellular or intracellular Ca2+. The results demonstrate an age-related change (1) in brain PKC activity and translocation and (2) in a physiological response to PKC stimulation. These results may have implications for other PKC-mediated functions that are altered during senescence.Keywords
This publication has 25 references indexed in Scilit:
- Influence of aging on catecholamine levels, accumulation, and release in F-344 ratsNeurobiology of Aging, 1987
- Selective alteration of mouse brain neurotransmitter release with ageNeurobiology of Aging, 1987
- Translocation of Protein Kinase C in Anterior Pituitary Tumor CellsJournal of Neurochemistry, 1987
- Aging Decreases the Sensitivity of Rat Cortical Synaptosomes to Calcium Ionophore‐Induced Acetylcholine ReleaseJournal of Neurochemistry, 1986
- The phorbol ester receptor: a phospholipid-regulated protein kinaseBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1985
- Phorbol ester and calcium act synergistically to enhance neurotransmitter release by brain neurons in cultureFEBS Letters, 1985
- Possible mechanisms involved in the release and modulation of release of neuroactive agentsNeurochemistry International, 1984
- 1-(5-isoquinol inesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase c in rabbit plateletsBiochemical and Biophysical Research Communications, 1984
- Age-dependent alterations of microtubule-associated enzyme activities from bovine brain (protein kinase, adenosine triphosphatase, guanosine triphosphatase)Mechanisms of Ageing and Development, 1983
- Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membraneNature, 1983