An Improved Method for Extracting Cell-Free Herpesviruses of Marek's Disease and Turkeys from Infected Cell Cultures

Abstract

Sonic extraction of cell-free Marek''s disease herpesvirus (MDHV) and turkey herpesvirus (HVT) from infected cell cultures was improved by incorporating sorbitol in the suspending media. Yields of cell-free virus of virulent MDHV were significantly increased with 10% sorbitol added to SPGA[sucrose-phosphate-glutamate-albumin]EDTA buffer. Avirulent strains of MDHV and HVT were, respectively, readily extracted with SPGA-EDTA and SPGA as the suspending medium, and extraction of their cell-free viruses was moderately improved by adding sorbitol to the suspending medium.