Toxoplasma gondii was isolated from the uterus of a 57-year-old white female who died of coronary thrombosis with no clinical or pathological evidence of current toxoplasmosis. The dye test titer was 1:256. The isolation technique was the digestion method of Jacobs and Melton (1957) which has been shown to destroy proliferative forms; the organisms were therefore believed to be encysted. The relation of this finding to the possibility of congenital transmission of toxoplasmosis from chronically infected mothers is discussed.