Development of large granular lymphocytes with anomalous, nonspecific cytotoxicity in clones derived from Ly-2+ T cells.
- 1 May 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (9), 2728-2732
- https://doi.org/10.1073/pnas.80.9.2728
Abstract
T cells cultured at limit dilution for 8 days in a concanavalin A-stimulated, filler-cell and growth factor-supported system produced cytolytic clones with high efficiency. These clones were not specific, lysing a wide range of targets, syngeneic and allogeneic, of tumor and normal cell origin. Lysis was a cell-mediated phenomenon but was not blocked by anti-Ly-2. One H-2-negative target was lysed, but one was resistant. Xenogeneic (human) tumor cells were not lysed. The cells in the clones were large, vacuolated, granular lymphocytes. They originated from single Ly-2+ responder cells and not from irradiated filler cells. Therefore, activated lymphocyte killers and other natural killer-like cells may be differentiated elements of the Ly-2+ T-cell lineage.Keywords
This publication has 12 references indexed in Scilit:
- Broadly reactive murine cytotoxic cells induced in vitro under syngeneic conditions.The Journal of Immunology, 1982
- Limit-dilution assay and clonal expansion of all T cells capable of proliferationJournal of Immunological Methods, 1982
- Semi-automated limit-dilution assay and clonal expansion of all T-cell precursors of cytotoxic lymphocytesJournal of Immunological Methods, 1982
- Studies of murine large granular lymphocytes. I. Identification as effector cells in NK and K cytotoxicities.The Journal of Immunology, 1982
- Propagation of mouse cytotoxic clones with characteristics of natural killer (NK) cellsCellular Immunology, 1982
- A new assay for cytotoxic lymphocytes, based on a radioautographic readout of 111In release, suitable for rapid, semi-automated assessment of limit-dilution culturesJournal of Immunological Methods, 1981
- Natural killer (NK) cells and their possible roles in resistance against disease.1981
- Fractionation, morphological and functional characterization of effector cells responsible for human natural killer activity against cell-line targetsCellular Immunology, 1979
- The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris, damaged cells and erythroid cells from lymphoid cell suspensionsJournal of Immunological Methods, 1972