Coronavirus JHM: Tryptic Peptide Fingerprinting of Virion Proteins and Intracellular Polypeptides

Abstract

The virion proteins and intracellular polypeptides of the murine coronavirus MHV-JHM were analyzed by 2-dimensional fingerprinting of their [35S]methionine-containing tryptic peptides. The analysis shows that the virion proteins gp98, gp65, pp60 and p23 are distinct. Virion protien gp25 has the same polypeptide component as p23, and virion protien pg170 has a polypeptide component related to gp98. The 6 virus polypeptides synthesized in infected [mouse sac(-)] cells, 150K, 65K, 60K, 30K, 23K and 14K are also distinct. The 170K and 98K species, which are produced by processing, are related to 150K. The 25K species is a processed form of 23K. The identity of corresponding species in the cell and in the virion has been shown and a model describing the genesis of coronavirus JHM proteins can now be proposed.