Novel carbenicillin-hydrolyzing β-lactamase (CARB-5) from Acinetobacter calcoaceticus var. anitratus
- 1 May 1989
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 59 (1-2), 45-50
- https://doi.org/10.1111/j.1574-6968.1989.tb03080.x
Abstract
A strain of Acinetobacter calcoaceticus var. anitratus highly resistant to ticarcillin but susceptible to ticarcillin in combination with clavulanic acid (2 mg/l) was found to produce a constitutive beta-lactamase. This enzyme was periplasmic with a characteristic substrate profile of a carbenicillin-hydrolyzing enzyme. Enzyme inhibition was detected with antiserum (anti-CARB-3), pCMB, cloxacillin, clavulanic acid and sulbactam. This novel enzyme with a molecular mass of 28,000 resembles other plasmid-mediated carbenicillinases (CARB) but differs in its apparent isoelectric point estimated as 6.3 and has been designated CARB-5 on this basis.Keywords
This publication has 18 references indexed in Scilit:
- Distribution of β-lactamases and phenotype analysis in clinical strains of Acinetobacter calcoaceticusJournal of Antimicrobial Chemotherapy, 1988
- Immunological comparison of constitutive β-lactamases of gram-negative bacteria by neutralization in zymogram gels: Properties of anti-TEM-1 and anti-TEM-2 seraAnnales de l'Institut Pasteur / Microbiologie, 1988
- Enzymatic resistance to β-lactams and aminoglycosides in Acinetobacter calcoaceticusJournal of Antimicrobial Chemotherapy, 1987
- Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel β-lactamaseJournal of Antimicrobial Chemotherapy, 1987
- Comparative evaluation of a new beta-lactamase inhibitor, YTR 830, combined with different beta-lactam antibiotics against bacteria harboring known beta-lactamasesAntimicrobial Agents and Chemotherapy, 1986
- An underestimated nosocomial pathogen, Acinetobacter calcoaceticusJournal of Antimicrobial Chemotherapy, 1985
- Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosaAntimicrobial Agents and Chemotherapy, 1983
- Properties of three carbenicillin-hydrolysing β-lactamases (CARB) from Pseudomonas aeruginosa: identification of a new enzymeJournal of Antimicrobial Chemotherapy, 1981
- Beta-Lactamases: Determination of Their Isoelectric PointsAntimicrobial Agents and Chemotherapy, 1978
- The Use of Analytical Isoelectric Focusing for Detection and Identification of -LactamasesJournal of General Microbiology, 1975