Properties of Structurally Restricted Antibody to Type VIII Pneumococcal Polysaccharide

Abstract

Following three courses of immunization with type VIII pneumococci, the serum of one rabbit showed an unusually marked increase in electrophoretically-restricted γ globulin components consisting of anti-polysaccharide antibodies. Examination of the intact purified antibodies by isoelectric focusing and of the corresponding light chains by disc gel electrophoresis in urea disclosed a less complex banding pattern than was observed with antibody isolated from serum of a rabbit showing a diffuse increase in globulin components after immunization. The serum concentration of the electrophoretically-restricted γ globulin components increased and subsequently decreased after each course of immunization. Histologic examination following the third course showed evidence of an intense immune response; plasma cell tumors were not seen.