Serial Passage of Human Tumors in Chick Embryo: Growth Inhibition by Nitrogen Mustard.

Abstract

This paper reports the successful serial passage of 3 human tumors: human epidermoid carcinoma no. 3 (H.Ep. no. 3), human sarcoma no. 1 (H.S. no. 1) and human embryonal rhabdomyosarcoma no. 1 (H. Emb. Rh. no. 1) on the chorioallantoic membrane and the H. Ep. no. 3 and H.S. no. 1 in the yolk sac. Metastases of the H.Ep. no. 3 to the livers and kidneys of the host chick were observed. The H.S. no. 1 and H.Ep. no. 3, when treated with a nitrogen mustard, HN2, showed the same pattern of histological injury previously described for the mouse sarcoma 180 growing in chick embryos and mice.