Ontogeny of synaptic phosphoproteins in brain.
- 1 August 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (8), 4037-4041
- https://doi.org/10.1073/pnas.75.8.4037
Abstract
The ontogeny of 2 endogenous substrates for cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase; EC 2.7.1.37) was studied in rat and guinea pig cerebrum. These endogenous substrates, referred to as proteins Ia and Ib, were shown in other studies to be specific to nervous tissue and to be enriched in the synaptic membrane and synaptic vesicle fractions of adult brain. In this present study, proteins Ia and Ib increased markedly during the time of major synaptogenesis in rat cerebrum and guinea pig cerebrum, in which the morphological development of synapses is predominantly postnatal and prenatal, respectively. Similar results were obtained by measuring endogenous phosphorylation of proteins Ia and Ib in the synaptic membrane fraction or by measuring phosphorylation of extracted proteins Ia and Ib with added protein kinase.This publication has 14 references indexed in Scilit:
- Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. II. Solubilization, purification, and some properties of an endogenous adenosine 3':5'-monophosphate-dependent protein kinase.Journal of Biological Chemistry, 1977
- Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. I. Solubilization, purification, and some properties of an endogenous phosphoprotein.Journal of Biological Chemistry, 1977
- Purification of phosphoprotein phosphatase from bovine cardiac muscle that catalyzes dephosphorylation of cyclic AMP-binding protein component of protein kinase.Journal of Biological Chemistry, 1977
- Widespread occurrence of a specific protein in vertebrate tissues and regulation by cyclic AMP of its endogenous phosphorylation and dephosphorylationMetabolism, 1975
- Synaptogenesis in guinea-pig cerebral cortex: A glutaraldehyte-PTA studyBrain Research, 1974
- Regulation of Endogenous Phosphorylation of Specific Proteins in Synaptic Membrane Fractions from Rat Brain by Adenosine 3′:5′-MonophosphateJournal of Biological Chemistry, 1973
- Synaptogenesis in the corpus striatum of infant ratExperimental Neurology, 1973
- The formation of synaptic junctions in developing rat brain: A quantitative electron microscopic studyBrain Research, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- The relationship between mammalian foetal weight and conception ageThe Journal of Physiology, 1951