R483 and F Plasmid Genes Promoting RNA Degradation: Comparative Restriction Mapping
- 1 September 1982
- journal article
- Published by Wiley in Microbiology and Immunology
- Vol. 26 (9), 779-793
- https://doi.org/10.1111/j.1348-0421.1982.tb00224.x
Abstract
The gene promoting nucleic-acid degradation (pnd) on IncIa plasmid R483 was cloned into pBR322. It is located on a 0.85 kilobase (kb) EcoRI-SalI fragment and is close to Tn7. The pnd gene has similar properties to the srnB gene on the F plasmid. A cleavage map of the 0.85 kb pnd fragment was constructed and compared with that of the 1.18 kb EcoRI-BamHI fragment containing the srnB gene. These two regions showed marked heterogeneity as evidenced by their distinctly different restriction maps. This result suggests separate paths of evolution of the two genes for stable RNA degradation.Keywords
This publication has 31 references indexed in Scilit:
- The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6S3Plasmid, 1981
- Map of plasmid RP4 derived by insertion of transposon CJournal of Molecular Biology, 1977
- Mapping of recognition sites for the restriction endonuclease from Escherichia coli K12 on bacteriophage PM2 DNAJournal of Molecular Biology, 1976
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- F Factor Promotes Turnover of Stable RNA in Escherichia coliScience, 1975
- Polynucleotide Sequence Relationships among Plasmids of the I Compatibility ComplexJournal of General Microbiology, 1974
- Plasmids Determining I Pili Constitute a Compatibility ComplexJournal of General Microbiology, 1973
- Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: II. Structure of drug resistance (R) factors and f factorsJournal of Molecular Biology, 1973
- R Factors Conferring Resistance to Trimethoprim but not SulphonamidesJournal of General Microbiology, 1972
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970