COMPARATIVE STUDIES OF AVIAN MYCOPLASMAS BY FLAT GEL POLYACRYLAMIDE ELECTROPHORESIS

Abstract

The phenol-acetic-acid extracted cell proteins of Mycoplasma (M.) and Acholeplasma (A.) reference strains PG31 (M. gallisepticum), PG16 (M. gallinarum), PG30 (M. iners), 17529 (A. laidlawii), CKK (Serovar C), DD (Serovar D), WR1 (Serogroup F), 695 (Serogroup I) and 694 (Serogroup L) were analyzed by the flat gel polyacrylamide electrophoresis. With the exception of PG8 and PG9, the Coomassie Blue-stained protein patterns show that each of the strains produced reproducible characteristic electrophoretic pattern by which the reference strains could be differentiated. Before the question could be answered whether the procedure described is suitable to replace the serological species differentiation of avian mycoplasmas, serological and electrophoretic studies of a relevant number of field strain are necessary.