Fluorescent protein tracers; a trial of new fluorochromes and the development of an alternative to fluorescein.
- 1 October 1958
- journal article
- Vol. 1 (4), 315-27
Abstract
With the object of simplifying the fluorescent protein tracer technique, the following fluorochromes were examined as possible alternatives to fluorescein: aminoeosin, aminorhodamine B, 3-phenyl-7-isocyanatocumarin (Geigy), 5-β-carboxyethylaminoacridine, R 4388 (Geigy), fluolite C (I.C.I.), lissamine flavine FFS (I.C.I.), lissamine rhodamine GS (I.C.I.), and lissamine rhodamine B 200 (I.C.I.) (RB 200). With the exception of RB 200, none was suitable as a protein label largely because of unsatisfactory fluorescence intensity or colour.Keywords
This publication has 6 references indexed in Scilit:
- Fluorescent protein tracers in the study of experimental liver damageThe Journal of Pathology and Bacteriology, 1958
- FLUORESCENT PROTEIN TRACERS A SIMPLE ALTERNATIVE TO FLUORESCEINThe Lancet, 1958
- Rapid Extraction with Ethyl Acetate of Free Fluorescein Derivatives from Fluorescein isoCyanate-Globulin ConjugatesNature, 1957
- Preserving Fluorescein Isocyanate for Simplified Preparation of Fluorescent AntibodyScience, 1957
- Distribution and elimination of 131I- and 14C-labelled plasma proteins in the rabbitBiochemical Journal, 1956
- Polarization of the fluorescence of macromolecules. 2. Fluorescent conjugates of ovalbumin and bovine serum albuminBiochemical Journal, 1952