STUDIES ON A NEW TYPE FLAVINNUCLEOTIDE

Abstract

It was found that an unknown flavin compound was synthesized from riboflavinylglucoside in the culture of Rhizopus oryzae. No liberation of riboflavin was observed during the culture. Using several solvent systems this new compound was chromatographically distinguishable from any known flavin compounds. Upon hydrolysis with H2SO4, flavin monophosphate-like substance was produced releaseing glucose. The enzymatic cleavage of this new compound by the phosphomonoesterase in Takadiastase produced riboflavinylglucoside and phosphate. Each one mole of glucose and phosphate per mole of this new compound was determined chemically. In the absorption spectrum there was no remarkable difference between the new compound and flavin mononucleotide. This new flavin compound was suggested to be a riboflavinylglucoside mononucleotide, whose phosphate was attached to one of the ribityl carbons other than 5[image]. The biosynthesis of flavin- mononucleotide-like substance from riboflavin by the same mole was also reported.