A simple method for sampling murine pulmonary and cardiac tissues for analysis of cyclic nucleotide levels
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Naunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie
- Vol. 303 (1), 55-61
- https://doi.org/10.1007/bf00496185
Abstract
A simple method for the rapid removal and freezing of mouse cardiac and pulmonary tissues is described. Samples thus obtained were judged to be suitable for valid estimation of in vivo levels of cyclic AMP and cyclic GMP based on the following findings: (a) the samples could be obtained and frozen in the very short time period of a few seconds; (b) no indication of adverse effects of the collection procedure was found upon examination of chemical indicators of energy metabolism; (c) the apparent rates of change of cyclic AMP and cyclic GMP levels during the first seconds after tissue isolation could produce small, but acceptable errors; and (d) dose-dependent elevations of pulmonary cAMP levels consistent with known effects in vitro were found after in vivo administration of isoproterenol.Keywords
This publication has 26 references indexed in Scilit:
- Pharmacologic blockade of the effect of histamine on lung cyclic AMP levels in normal and pertussis-vaccinated miceBiochemical Pharmacology, 1977
- Pulmonary patterns of adenosine-3′, 5′-cyclic monophosphate accumulations in response to adrenergic or histamine stimulation in Bordetella pertussis-sensitized miceBiochemical Pharmacology, 1976
- Effects of decapitation, ether and pentobarbital on guanosine 3′,5′-phosphate and adenosine 3′,5′-phosphate levels in rat tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Rate of inactivation of adenyl cyclase and phosphodiesterase: Determinants of brain cyclic AMPLife Sciences, 1974
- Regulation by β-adrenergic receptor and muscarinic cholinergic receptor activation of intracellular cyclic AMP and cyclic GMP levels in rat lung slicesBiochemical and Biophysical Research Communications, 1973
- THE USE OF MICROWAVE HEATING TO INACTIVATE CHOLINESTERASE IN THE RAT BRAIN PRIOR TO ANALYSIS FOR ACETYLCHOLINEJournal of Neurochemistry, 1973
- FREEZE‐BLOWING: A NEW TECHNIQUE FOR THE STUDY OF BRAIN IN VIVOJournal of Neurochemistry, 1973
- Cyclic AMP in brain areas: Effects of amphetamine and norepinephrine assessed through the use of microwave radiation as a means of tissue fixationBrain Research, 1972
- REGIONAL ENERGY RESERVES IN MOUSE BRAIN AND CHANGES WITH ISCHAEMIA AND ANAESTHESIA*Journal of Neurochemistry, 1966
- SUBSTRATE CHANGES IN PERIPHERAL NERVE DURING ISCHAEMIA and WALLERIAN DEGENERATION*Journal of Neurochemistry, 1965