A CYTOCHEMICAL LOCALIZATION OF REDUCTIVE SITES IN A GRAM-NEGATIVE BACTERIUM

Abstract

In order to obtain information on the exact location of the respiratory enzyme chain in Gram-negative bacteria, an electron microscopic study was made of the sites of reducing activity of cells that had, in the living state, incorporated tellurite. In the test object Proteus vulgaris, the reduced tellurite was found to be deposited in bodies contiguous with the plasma membrane but different in structure from those described in the Gram-positive Bacillus subtilis (2). In fact, the bodies proved to consist of a conglomerate of elements which contained the strongly electron-scattering reduced tellurite and a delicately granular "matrix." A limiting membrane was not observed around these complexes. In serial sections details of the complexes are illustrated. Reduced tellurite was not deposited in the plasma membrane to any important degree. Since no other sites of deposition of the reduced product were revealed, it is assumed that the complexes represent the mitochondrial equivalents in the investigated organism. In addition, the bodies might function as the basal granules of the flagella.