Available lysine. I.—Determination of non‐N‐terminal lysine in protein
- 1 September 1968
- journal article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 19 (9), 492-495
- https://doi.org/10.1002/jsfa.2740190903
Abstract
An improved quantitative method for estimating ϵ‐DNP‐lysine is described. This method is applied to the determination of ϵ‐DNP‐lysine produced by the hydrolysis of dinitrophenylated proteins. The effect of the conditions of hydrolysis and of traces of chromic acid present during hydrolysis is presented. The use of dinitrophenylation as a method of determining non‐N‐terminal lysine was tested on three purified proteins and the results are discussed.This publication has 16 references indexed in Scilit:
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