The Nucleotide Sequence Complexity of Avian Tumor Virus RNA
- 1 September 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9), 3560-3564
- https://doi.org/10.1073/pnas.71.9.3560
Abstract
The nucleotide sequence complexity of 60-70S RNA of Rous sarcoma virus was determined from the molar yields of 11 pure oligonucleotides of known chain length, obtained from [32P]RNA of Rous sarcoma virus by T1 RNase digestion and two-dimensional polyacrylamide gel electrophoresis. We calculate an apparent chain length of about 9800 nucleotides, corresponding to a molecular weight of 3.4 × 106. Assuming the molecular weight of 60-70S RNA of Rous sarcoma virus to be about 107, our data suggest that the virus genome consists of identical, or very similar, nucleotide sequences repeated three to four times.Keywords
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