Biosynthesis and Composition of Histones in Novikoff Hepatoma Nuclei and Nucleoli
- 22 April 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 152 (3721), 521-523
- https://doi.org/10.1126/science.152.3721.521
Abstract
Histones were prepared from isolated nuclei and nucleoli of the Novikoff ascitic hepatoma at several time points after the injection of L-lysine uniformly labeled with C14 into tumor-bearing rats. Amino acid analysis and starch-gel electrophoresis failed to reveal any differences between the nuclear and nucleolar histones, although both fractions were more acidic in composition than calf thymus histones. However, the nucleolar histones were a metabolically distinct fraction, and their rate of synthesis was approximately twice that of the total nuclear histones.This publication has 6 references indexed in Scilit:
- Studies on nuclear proteins II. Quantitative distribution of histone fractions in various tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- Intranuclear Site of Histone SynthesisScience, 1964
- Nucleolus: A Center of RNA MethylationScience, 1963
- TRANSFER OF THE METHYL GROUP OF METHIONINE TO NUCLEOLAR RIBONUCLEIC ACIDBiochemical Journal, 1963
- PROTEIN SYNTHESIS BY ISOLATED PEA NUCLEOLIThe Journal of cell biology, 1963
- The metabolism of isolated rat-liver nucleoli and other subnuclear fractions. The active site of amino acid incorporation in the nucleusBiochemical Journal, 1963