Preparation and Culture of Spirogyra and Zygnema Protoplasts

Abstract

Protoplasts were enzymatically released from Spirogyra and Zygnema. The released protoplasts cultivated in liquid media regenerated a wall, grew in filamentous fashion and usually divided with an incomplete septum. When the media osmolarity was gradually reduced, the regenerated cells grew further. These regenerated cells appeared to be the same as the original cells.