Primary in Vitro Cytotoxic Response of F1 T Lymphocytes against Parental Antigens

Abstract

Spleen cells from adult female (AKR/J × BALB/c)F₁ mice can respond to mitomycin C-treated spleen from AKR/J mice and can generate effector CTL in a 5-day primary in vitro culture. The response is comparable in magnitude to the response to allogeneic H-2K or H-2D antigens. The response is T cell mediated and is directed to antigen(s) present only on the parental cells. The target cell must be homozygous at H-2K^k to be lysed and H-2D^k antigens do not serve as a target in this response. Spleen cells from (B10.BR × B10.D2) hybrids that have been stimulated with AKR/J lyse B10.Br as well as AKR/J target cells. Similar H-2^k/d hybrid F₁ anti-H-2^k parent responses are seen in certain other strain combinations. A number of possible interpretations of these responses are discussed.