Cholesterol metabolism. 5. The excretion of acids after cholesterol ingestion in rats

Abstract

The fecal excretion of acids in rats fed low-fat and added fat (16.6%) diets containing small amounts or large amounts (1.6%) of cholesterol was investigated. The amounts of ether-extractable acids and of ester cholesterol are increased after sterol feeding. The ether-extractable acids were separated into solid and liquid acid fractions, converted into methyl esters and fractionally distilled. The light petroleum-insoluble compounds were separated from the fractions. Analyses of the acids are given; these show that the increases after cholesterol feeding occur in the solid, liquid and light petroleum-insoluble fractions. The methyl esters of the light petroleum-insoluble esters were chromatographed and gave a number of fractions, some of which are probably ketonic bile acids.