Role of Magnesium in the Binding of Substrate and Effectors to Phosphoenolpyruvate Carboxylase from a CAM Plant
- 1 June 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 87 (2), 443-446
- https://doi.org/10.1104/pp.87.2.443
Abstract
The binding of phosphoenolpyruvate, malate, and glucose 6-phosphate to phosphoenolpyruvate carboxylase purified from Crassula argentea Thunb. was measured using both the intrinsic tryptophan fluorescence of the enzyme and the extrinsic fluorescence of the complex of 8-anilino-1-napthalenesulfonate with the enzyme. It was found that the substrate phosphoenolpyruvate can bind in the absence of magnesium but is bound in greater quantities and more tightly when magnesium is present. Malate reduces the binding of phosphoenolpyruvate, while glucose 6-phosphate increases the binding of the substrate. Glucose 6-phosphate requires magnesium to bind to the enzyme, while malate does not. The general trends from the binding experiments using fluorescence methods were confirmed by activity determinations using assays performed in the absence of magnesium.This publication has 9 references indexed in Scilit:
- A Simple and Accurate Spectrophotometric Assay for Phosphoenolpyruvate Carboxylase ActivityPlant Physiology, 1988
- Regulation of Phosphoenolpyruvate Carboxylase from Crassula argenteaPlant Physiology, 1987
- Malate Inhibition of Phosphoenolpyruvate Carboxylase from CrassulaPlant Physiology, 1986
- Purification of the phosphorylated night form and dephosphorylated day form of phosphoenolpyruvate carboxylase from Bryophyllum fedtschenkoiBiochemical Journal, 1986
- Regulation of phosphoenolpyruvate carboxylase from Crassula by interconversion of oligomeric formsArchives of Biochemistry and Biophysics, 1985
- Diurnal Regulation of Phosphoenolpyruvate Carboxylase from CrassulaPlant Physiology, 1985
- Kinetic Analysis of effectors of phosphoenolpyruvate carboxylase from Bryophyllum fedtschenkoiBiochimica et Biophysica Acta (BBA) - Enzymology, 1980
- Spinach leaf phosphoenolpyruvate carboxylase: Purification, properties, and kinetic studiesArchives of Biochemistry and Biophysics, 1974
- The enzymatic carboxylation of phosphoenolpyruvate. IV. The binding of manganese and substrates by phosphoenolpyruvate carbosy-kinase and phosphoenolypyruvate carboxylase.1968