Immunohistochemical Localization of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase and Myelin Basic Protein in the Central Nervous System of the Jimpy and the Normal Mouse
- 1 March 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (3), 686-691
- https://doi.org/10.1111/j.1471-4159.1985.tb12869.x
Abstract
The immunohistochemical localization of 2'',3''-cyclic nucleotide-3''-phosphodiesterase (CNPase) and myelin basic protein (MBP) in CNS of the jimpy mutant mouse which is characterized by dys- and demyelination is described. In controls, the CNPase and MBP were localized exclusively in white matter in the CNS. The jimpy mutant mice were severely affected: A very weak reaction was observed in the white matter. Very few CNPase- and MBP-positive myelin sheaths were observed and some degradation products were also observed after reaction with antisera both CNPase and MBP. The immunohistochemical reaction in the jimpy mice showed a similar localization in both CNPase and MBP.Keywords
This publication has 25 references indexed in Scilit:
- Immunochemical Identification of 2′, 3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in Central and Peripheral Nervous System Myelin, the Wolfgram Protein Fraction, and Bovine OligodendrocytesJournal of Neurochemistry, 1983
- Immunohistochemical Localization of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase and Myelin Basic Protein in the Chick RetinaJournal of Neurochemistry, 1983
- Intracellular Localization of 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in a Neuronal Cell Line as Examined by Immunofluorescence and Cell FractionationJournal of Neurochemistry, 1981
- Characterization of 2′:3′‐Cyclic Nucleotide 3′‐Phosphodiesterase: Limited Proteolytic Digestion, Plant Lectin Affinity Chromatography, and Immunological IdentificationJournal of Neurochemistry, 1981
- Subcellular Distribution and Developmental Change of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase in the Central Nervous System of the Myelin‐Deficient Shiverer Mutant MiceJournal of Neurochemistry, 1980
- A Rapid Micro Method for 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase Assay Using Micro High Performance Liquid ChromatographyJournal of Neurochemistry, 1980
- Improved Method for Prufication of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase from Bovine Cerebral White MatterJournal of Neurochemistry, 1980
- PURIFICATION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE FROM BOVINE BRAIN BY IMMUNOAFFINITY CHROMATOGRAPHY: FURTHER BIOCHEMICAL CHARACTERIZATION OF THE PROTEINJournal of Neurochemistry, 1979
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978
- Carbodiimides. VIII.1 Observations on the Reactions of Carbodiimides with Acids and Some New Applications in the Synthesis of Phosphoric Acid EstersJournal of the American Chemical Society, 1958