Serotonin transport by cultured bovine aortic endothelium.
- 1 June 1975
- journal article
- abstracts
- Published by Wolters Kluwer Health in Circulation Research
- Vol. 36 (6), 799-806
- https://doi.org/10.1161/01.res.36.6.799
Abstract
Endothelial cells isolated from bovine aortas without prior treatment with enzymes were cultured in RPMI 1640 medium containing 17% fetal calf serum and antibiotics. The endothelial cells at confluency (7 days) were similar to endothelium in situ or to freshly isolated endothelial cells from blood vessels as seen by light, scanning, and electron microscopy. Cultured and freshly isolated indothelial cells exposed to labeled serotonin, even in the presence of iproniazid (5 x 10-4M), took up approximately 125 and 250 pmoles 14C-serotonin/mg protein, respectively, in 3 hours. Imipramine (10-4M) reduced uptake for both cell groups. Cold (4 degrees C) and metabolic inhibitors sharply reduced serotonin uptake by both freshly isolated and cultured endothelial cells. Ouabain (10-5M) almost completely blocked serotonin transport. Six analogues of serotonin at concentrations ten times above experimental serotonin concentrations did not affect serotonin transport in the cultured endothelial cells but did reduce it in the freshly isolated endothelial cells by 50%. The data on transport suggest that serotonin uptake is not unique to pulmonary endothelium, as has been suggested previously. In addition, using cultured indothelial cells to study serotonin transport is compatible with using other serotonin model systems such as platelets, lung, or brain. Lastly, serotonin uptake by endothelial cells may involve an active transport mechanism similar to that described for the pulmonary circulation, platelets, and insect salivary glands.Keywords
This publication has 17 references indexed in Scilit:
- DNA synthesis in rat aortic endothelium: Effect of bacterial endotoxin and traumaMicrovascular Research, 1974
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973
- Replication of Human Endothelial Cells in CultureScience, 1973
- Isolation and growth characteristics of cell lines from bovine venous endotheliumIn Vitro Cellular & Developmental Biology - Plant, 1973
- GAP JUNCTIONS IN ARTERIAL ENDOTHELIUMThe Journal of cell biology, 1973
- RADIOAUTOGRAPHIC DEMONSTRATION OF 5-HYDROXYTRYPTAMINE-3H UPTAKE BY PULMONARY ENDOTHELIAL CELLSThe Journal of cell biology, 1972
- Metabolic Fates of Bradykinin, Angiotensin I, Adenine Nucleotides and Prostaglandins E1 and F1α in the Pulmonary CirculationPublished by Springer Nature ,1972
- Platelet-microvasculature interaction during one passage through the dog lungMicrovascular Research, 1971
- THE EFFECTS OF ENDOTOXIN ON VASCULAR ENDOTHELIUMThe Journal of Experimental Medicine, 1969
- The release and fate of vaso‐active hormones in the circulationBritish Journal of Pharmacology, 1969