Production and partial characterization of monoclonal antibodies to Mycobacterium leprae
- 1 July 1982
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 37 (1), 172-178
- https://doi.org/10.1128/iai.37.1.172-178.1982
Abstract
Monoclonal antibodies to M. leprae were produced by the fusion of BALB/c (mouse) splenocytes and lymph node cells to BALB/c myeloma (NSI/1) cells. Eleven monoclonal antibodies were characterized as to their reactivity with M. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay [ELISA] and immunofluorescence. Two monoclonal antibodies reacted only with M. leprae; the other 9 showed unique patterns of reactivity by ELISA. One monoclonal antibody (IIH9) reacted with a 68,000-dalton protein present in extracts from M. leprae, M. tuberculosis H37Rv, M. gastri and M. smegmatis. Potential uses for these antibodies in serological tests and immunochemical analyses are discussed.This publication has 22 references indexed in Scilit:
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