FEULGEN NUCLEAL REACTION III. A CONTRIBUTION TO THE STUDY OF THE ADSORPTION ISOTHERM AND STOICHIOMETRY OF FEULGEN'S NUCLEAL REACTION IN SITU

Abstract

To gain some insight into the staining mechanism of the Feulgen nucleal reaction in situ, the staining reaction of 3 kinds of hydrolyzed nucleoprotein-containing films [Scomberomorus niphonius deoxyribonucleoprotein, calf thymus deoxyribonucleohistone and commercial egg albumin] with Schiff''s reagent was studied from physicochemical and chemical view points. The establishment of an approximate equilibrium state in staining was assumed, and the mode of the adsorption isotherm in this state was examined. The Feulgen nucleal reaction in the heterogeneous phase conforms to Langmuir''s adsorption isotherm and thus exhibits a saturation value in staining. Based on this fact, the stoichiometry of the reaction was determined. The saturation value of the stoichiometry was proved to be 4.