Deoxyribonucleic Acid Methylase Activity in Pea Seedlings
- 9 May 1969
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 164 (3880), 721-722
- https://doi.org/10.1126/science.164.3880.721
Abstract
Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30 degrees C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.Keywords
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