Stretch-activated ion channels modulate the resting membrane potential during early embryogenesis
- 22 October 1988
- journal article
- research article
- Published by The Royal Society in Proceedings of the Royal Society of London. B. Biological Sciences
- Vol. 235 (1278), 95-102
- https://doi.org/10.1098/rspb.1988.0064
Abstract
By using the patch-clamp technique, stretch-activated ionic channels were found in the membrane of cleaving freshwater fish embryos at the early stages of embryogenesis (2-256 cells). The application of negative pressure to the pipette increased the frequency of activation and the duration of bursts. This type of channel has a preferential K$^{+}$ selectivity. When bathed on both membrane surfaces with 140 mM KCl the channel conductance was 71 pS. The kinetic behaviour did not depend markedly on either membrane potential (in the range from -70 to +70 mV) or calcium concentration on the cytoplasmic side of the membrane. On continuous recording, the probability of the channel being open was found to change periodically over a 5- to 20-fold range for different cells. These variations correlated with changes in resting potential and membrane conductance during the cell cycle. These results suggest that the oscillation of resting potential within the cell cycle is associated with the operation of stretch-activated ion channels.Keywords
This publication has 20 references indexed in Scilit:
- Mediation of cell volume regulation by Ca2+ influx through stretch-activated channelsNature, 1987
- Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers?Nature, 1987
- Depolymerization of microtubules alters membrane potential and affects the motional freedom of membrane proteinsBiochemistry, 1986
- Stretch‐activated single ion channel currents in tissue‐cultured embryonic chick skeletal muscle.The Journal of Physiology, 1984
- Mechanoelectrical Transduction by Hair Cells in the Acousticolateralis Sensory SystemAnnual Review of Neuroscience, 1983
- Observations on intracellular pH during cleavage of eggs of Xenopus laevis.The Journal of cell biology, 1981
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.Proceedings of the National Academy of Sciences, 1980
- Mechanical properties of the surface of the sea urchin egg at fertilization and during cleavage*1Experimental Cell Research, 1974
- Depolarization of sensory terminals and the initiation of impulses in the muscle spindleThe Journal of Physiology, 1950