Two control regions for eukaryotic tRNA gene transcription.
- 1 June 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (6), 3365-3368
- https://doi.org/10.1073/pnas.77.6.3365
Abstract
Two Drosophila tRNALys genes with identical coding sequences transcribe with very different efficiencies in nuclear extracts from Xenopus oocytes. The use of recombinant plasmids in which the 5''-flanking sequences of these genes were either switched or replaced by defined [Escherichia coli] pBR322 sequences revealed 2 control regions for tRNA gene transcription. An internal control region comprising the mature tRNA coding sequence (and possibly its 3''-flanking sequences) is sufficient for transcription initiation, and an external control region comprising the 5''-flanking sequences represses this transcription. All transcripts have short leader sequences. Altered precursor tRNA transcribed from truncated tRNALys genes (missing a single base pair in the acceptor stem) are not processed well in vitro.This publication has 24 references indexed in Scilit:
- Analysis of a drosophila tRNA gene clusterCell, 1980
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Transcription of a cloned bombyx mori trna2Ala gene: Nucleotide sequence of the tRNA precursor and its processing in vitroCell, 1979
- In vitro transcription and processing of a yeast tRNA gene containing an intervening sequenceCell, 1979
- Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytesNature, 1979
- Deletion mutants of Xenopus laevis 5S ribosomal DNACell, 1979
- A nuclear extract of xenopus laevis oocytes that accurately transcribes 5S RNA genesCell, 1978
- Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structuresJournal of Molecular Biology, 1975