Zone Electrophoresis Studies on Hemagglutinin of Hemagglutinating and of Masked Strain of Polyoma Virus

Abstract

Polyoma virus hemagglutinin of a hemagglutinating strain (1000) and of a masked strain (8844) were examined by zonal electrophoresis in a density gradient of cane sugar. The masked strain 8844 was unmasked by heat treatment previous to electrophoresis. Both hemagglutmins migrated with the same speed, slightly faster than rabbit hemoglobin, when tested after an electrophoresis for 3 hrs. at pH 8.6. Support for the presence of an inhibitor of hemagglutinin in the masked strain 8844 gained. It appeared that a hemagglutinin-inhibitor separation could be achieved when previously unmasked hemagglutinin was purified by electrophoresis.