Specific cleavage of tRNA by nuclease S1
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6), 865-872
- https://doi.org/10.1093/nar/2.6.865
Abstract
Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5′ phosphate and 3′ OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.Keywords
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