Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

Abstract

Satellite RNA of CARNA 5 (cucumber mosaic virus-associated RNA 5) emerged in serial passage lines of Spanish cucumber mosaic virus isolates established in Chenopodium, squash and 2 tobacco varieties. No satellite was detected in virus obtained from tomato serial passage lines. The satellites were identified by their biological effect on tomato, by their gel electrophoretic morbidity and by nucleic acid hybridization, as being very similar in sequence to known CARNA 5. Interactions among host, virus and CARNA 5 affect the selection of a particular CARNA 5 variant.